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==Product purification== *Purify the amplified library with QIAquick column. **Take 20uL sample and eluted with 15uL EB. *Gave the purified libraries to Alice for Solexa sequencing. Lib. name Sample Sequencing tag ---------------------------------------------------------- sam003 10-1 s_1, HC-MDA-hCell-Dec17-sam003 sam004 10-2 s_2, HC-MDA-hCell-Dec17-sam004 sam005 11-1 s_3, HC-MDA-hCell-Dec17-sam005 sam006 11-2 s_7, HC-MDA-hCell-Dec17-sam006 *Stored the rest of unpurified samples in -20C. '''NOTE: *For the qPCR amplification. I also tried the newly synthesized primer(PCR_F and PCR-R). Although the templates were succesuflly amplified, the amplification curves were not consistent with the TBE-PAGE results. *I am wondering if my qPCR didn't perform well on the final vortexing and spining step. There may have some reaction sticked to the tube cap. **The current results were completed using the original qPCR primer (From Dr. Zhang) by a repeat qPCR reaction. **I will need to try the qPCR amplification on those newly synthesized primer again. ===Solexa sequence result analysis=== '''The normalized enrichment(%) of MDA amplification''' [[File:sam012410-Solexa seq results.jpg|1100px]] *In general, the two step amplification (1hr followed by 10hr) showed more serious amplification bias with higer Stdev.
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