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=== Test #2 (BBI_E4_T2) === *Date: 4/17/13 ~ *Cells: P11 H9Bb3 derived osteoblasts. Feeder free culture system. Cell seeded on 4/17 in three glass bottom wells and started osteogenic differentiation (5% serum osteogenic medium) on 4/20. *Viruss: Adenoviruses: Ad-CreERT2, 10e10 PFU/ml *Observations: After osteogenic differentiation, cells remain proliferating for a while. In order to increase transfection efficiency, I wait for a few more days until these cells stop dividing (on 4/26, 6 days after induction). *Infection on 4/26: (1)Prepare virus-containing media: {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Group''' | align="center" style="background:#f0f0f0;"|'''amount of virus (ul/ 1 ml medium)''' | align="center" style="background:#f0f0f0;"|'''MOI (multiplicity of infection)''' |- | A ||1||10 |- | B ||5||50 |- | C ||10||100 |- |} (2)Aspirate original culture media. Add 1ml virus-containing media to each glass-bottom wells.<br> (3)Incubate for 24hrs at 37C. Remove virus-containing media and replace it with fresh osteogenic induction media.
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