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====Template Not Digested Notes==== *Theoretically should be the same? Since RNA template cannot be amplified by KAPA polymerase and thermocycling should denature padlock probes from template **Maybe PCR primers are occupied with template *Separation between sample and NTC for 820ng and 30ng almost non-existent but 150ng still has good separation **I could see more template leads to PCR primers being occupied on template, which is why 820ng sample amplifies less than NTC but I would then expect 150ng to show more similarity to that than 30ng *PosCtrl and NegCtrl look "correct"
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