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=== Things to check === : - Beta-mercaptoethanol --> Yes : - Magnetic rack for 1.5mL tube is working well? --> Yes : - Need to use carriers? : - ZymoBeads allows for 50ul samples. Do I need to do multiple tubes for total 200ul sample? Double check with Dr. Zhang the total volume required to test for each kit. : - How to combine DNA purification with bisulfite conversion for bead-based methods. :: '''Recommended paper''' from Dr. Zhang: [[Media:Bailey_VJ_etal2010.pdf|Bailey VJ et al, 2010 ]] :: - In this paper, the step of cell lysis and bisulfite conversion were performed in the same tube and used the same magnetic beads in both steps. The house made buffers of bisulfite conversion step were prepared to compatible with the magnetic beads used in this experiment. :: - [[Media:BaileyVJ_etal2010_Supp_Methods.pdf|BaileyVJ_etal2010_Supp_Methods]] ==== 2014-03-04 ==== : - Received 3 case samples from Sharmeela :{| {{table}} class = wikitable | align="center" style="background:#f0f0f0;"|'''Case/Accession #''' | align="center" style="background:#f0f0f0;"|'''Project ID #''' | align="center" style="background:#f0f0f0;"|'''Typr/Amount''' | align="center" style="background:#f0f0f0;"|'''Daigonosis''' |- | AA005||UCSD004.1||4-BC,P,S||Eosinophilia |- | AA007||UCSD004.2||4-BC,P,S||Anemia |- | AA008||UCSD004.3||4-BC,P, 3S||Anemia |} :: Note: SampleUCSD004.3 had only 3 aliquots of serum :: - White caps : Buffy coat (BC) :: - Green caps: Plasma (P) :: - Blue caps : Serum (S) ==== 2014-03-10 ==== '''Discussion with Dr. Zhang'''
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