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===Plate operation:=== '''precoating (modified)''' 1. Prepare Matrigel (coating solution, thaw at 4C, on ice, overnight). 2. Aspirate solution from wells 1 and 8. Leave bottom holes fill with PBS. 3. Aspirate PBS from well 6 and 7, including bottom hole. 4. Add 20ul coating solution to well 6 (add to the bottom hole). 5. Incubate at room temperature for 3 hrs. 6. Wash step: aspirate coating solution from well 6 and 7. Add 10ul culture medium to the bottom hole of well 6. Incubate at room temperature for another 3 hrs.
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