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==EtOH precipitation== *Transfer the amplified amplicon from 96-well plate into 50-mL disposable basins (Total volume ~9.6mL) *Aliquot 1.2 mL of amplified amplicon into 15-mL tube (4 tubes) *In each tube, add 4 ul of Glycoblue, 120 ul of 3M NaOAc, and 3 mL of 100 % EtOH (2.5 volume of 100% EtOH, 1/300 volume of Glycoblue, 0.1 volume of 3M NaOAc pH 5.2 or 5.5) *Mix well and leave at -80C for at least 30 min. *Precipitate by centrifugation at ~10,000xg for 30 min, at 4 C *Discard spnt and add 800ul of cold 75% EtOH to each tube. *Use 1 mL clean pipette tip transfer DNA pellet to 1.5 mL tube. *Rinse the pellet and spin the tube at 14,000 rpm for 5 min. *Discard spnt and air-dry the pellet for 3-5 min in the hood (PCR work station). *Resuspend DNA with 150 ul ddH2O mix well by pipetting up and down.
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