Editing
Jie:LabNotes/CpgSeq/2008-8-1
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Treat circlehelper with Antarctic phosphatase 2008-08-21== circlehelper_V4.2_2_Ida (100uM) 2.4ul 10x Antarctic phosphatase buffer 1ul Antarctic phosphatase (5U/ul) 2ul ddH2O 5ul 37C 15mins -> 65C 5mins. Annealing system is setup is as follows: tube1 template 6ul 10x Ampligase Buffer 1ul Treated Circlehelper(100uM) 1ul dNTP(10mM) 0.5ul Stoffel fragment(10U/ul) 0.5ul Ampligase 1ul tube1:94C 3min -> 5 cycles X (94C 1 min -> 60C 10mins) -> 4C . tube2:94C 3min -> 5 cycles X (94C 1 min -> 60C 10mins) -> 95C 1min ->add ExoI/III 2ul -> 37C 1h -> 95C 5mins -> 4C. PAGE gel purification. Elute in 11ul ddH2O. add 1.5ul 10x NEB Buffer 2, 2ul AluI_V6 oligo (100uM) 95C 10mins -> turn off the machine and wait for 20mins. -> add AluI 2ul ->37C 1h.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information