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==== 8/01/2013 ==== * From sequencing result, seem like we didn't get a good result, and we need to repeat capture. * I summarized the total volume/amount/concentration of gDNA samples we received from BP project. {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Sample''' | align="center" style="background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Volume for 500ng''' | align="center" style="background:#f0f0f0;"|'''WellIDs''' | align="center" style="background:#f0f0f0;"|'''Volume left (ul)''' | align="center" style="background:#f0f0f0;"|'''Total volume (ul)''' | align="center" style="background:#f0f0f0;"|'''Total amount (ng)''' | align="center" style="background:#f0f0f0;"|'''gDNA left''' | align="center" style="background:#f0f0f0;"|'''gDNA repeat''' | align="center" style="background:#f0f0f0;"|'''Bis-cvt left''' | align="center" style="background:#f0f0f0;"|'''bis-cvt repeat''' | align="center" style="background:#f0f0f0;"|'''Bad qual''' |- | 1||37.71||13.26||A1||5.80||19.06||718.74||218.74||y||123.00||y|| |- | 2||39.43||12.68||B1||6.20||18.88||744.46||244.46||y||167.20||yy|| |- | 3||50.57||9.89||C1||9.50||19.39||980.43||480.43||yy||98.30||y|| |- | 4||34.86||14.34||D1||5.00||19.34||674.29||174.29||||143.80||yy||x |- | 5||56.00||8.93||E1||9.50||18.43||1032.00||532.00||yy||117.80||y|| |- | 6||44.29||11.29||F1||8.00||19.29||854.29||354.29||y||125.60||yy||x |- | 7||73.71||6.78||G1||12.00||18.78||1384.57||884.57||yy||136.00||yy|| |- | 8||29.71||16.83||H1||3.50||20.33||604.00||104.00||||138.60||yy|| |- | 9||40.29||12.41||A2||8.00||20.41||822.29||322.29||y||117.80||y|| |- | 10||33.43||14.96||B2||12.50||27.46||917.86||417.86||yy||162.00||yy||x |- | 11||44.86||11.15||C2||8.50||19.65||881.29||381.29||y||97.78|||| |- | 12||52.86||9.46||D2||10.00||19.46||1028.57||528.57||yy||94.14|||| |- | 13||11.34||9.00||E2||0.00||9.00||102.09||0.00||||0.00||||x |- | 14||36.86||9.00||F2||0.00||9.00||331.71||0.00||||0.00||||x |- | 15||26.74||9.00||G2||0.00||9.00||240.69||0.00||||0.00|||| |- | 16||50.00||9.00||H2||0.00||9.00||450.00||0.00||||10.16||||x |- | 17||34.00||14.71||A3||22.00||36.71||1248.00||748.00||yy||0.28||||x |- | 18||31.14||16.06||B3||22.00||38.06||1185.14||685.14||yy||9.12||||x |- | 19||26.46||18.90||C3||22.00||40.90||1082.06||582.06||yy||0.00||||x |- | 20||20.29||20.00||D3||18.50||38.50||781.00||375.29||y||0.00||||x |- | 21||18.23||20.00||E3||15.50||35.50||647.11||282.54||y||0.00||||x |- | 22||29.43||16.99||F3||22.00||38.99||1147.43||647.43||yy||0.00||||x |- | 23||47.71||10.48||G3||22.00||32.48||1549.71||1049.71||yy||31.48|||| |- | 24||32.57||15.35||H3||23.00||38.35||1249.14||749.14||yy||106.36||y|| |- | 25||29.14||17.16||A4||21.00||38.16||1112.00||612.00||yy||125.60||yy|| |- | 26||19.60||20.00||B4||18.00||38.00||744.80||352.80||y||149.00||yy||x |- | 27||24.31||20.00||C4||19.00||39.00||948.26||461.97||yy||159.40||yy|| |- | 28||23.66||20.00||D4||19.00||39.00||922.63||449.49||yy||258.20||yy|| |- | 29||47.43||10.54||E4||9.00||19.54||926.86||426.86||yy||120.40||y||x |- | 30||76.86||6.51||F4||12.50||19.01||1460.71||960.71||yy||0.00||||x |- | 31||40.00||12.50||G4||7.00||19.50||780.00||280.00||y||461.00||yy|| |- | 32||32.29||15.49||H4||4.00||19.49||629.14||129.14||||578.00||yy||x |} * Total amount is the amount of gDNA we got ** 4 of them (13-16) have total amount < 500ng and we don't have more gDNA/bis-cvt gDNA to repeat experiment. We may need to request more if that's possible and Dr. Zhang agree to do that. * gDNA repeat and bis-cvt repeat mean the both gDNA and bis-cvt gDNA are enough to repeat ** y = lower than required, but expected to be enough ** yy= equal or more than enough to repeat * I am going to do bis-cvt for all samples (except 4 samples) and summarize the list of sample that need to request more if possible.
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