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===(ISB_CA_AF and ISB_CA_AR_T2 for ppMALTA1) stored in #18, #19 Alan Misc. -20C=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> |- style="font-size:12pt;font-weight:bold" align="center" | width="111" height="15" | Probes | width="77" | BSPP_GP1_V6 | width="65" | illumina_155 |- style="font-size:12pt;font-weight:bold" align="center" | height="15" | Sample | Jurkat_CT | Jurkat_CT |- style="font-size:12pt;font-weight:bold" align="center" | height="15" | Index | align="center" | 1 | align="center" | 2 |- style="font-size:12pt" align="center" | height="15" | Components | 1x rxn | 1x rxn |- style="font-size:12pt" align="center" | height="15" | Captured Template | align="center" | 20 | align="center" | 20 |- style="font-size:12pt" align="center" | height="15" | 10uM AmpF6.4Sol | align="center" | 1 | align="center" | 1 |- style="font-size:12pt" align="center" | height="15" | 10uM AmpR6.3Indx | align="center" | 1 | align="center" | 1 |- style="font-size:12pt" align="center" | height="15" | 2X KAPA SYBR MM | align="center" | 25 | align="center" | 25 |- style="font-size:12pt" align="center" | height="15" | H2O | align="center" | 3 | align="center" | 3 |- style="font-size:12pt" align="center" | align="center" height="15" | | align="center" | | align="center" | |- style="font-size:12pt;font-weight:bold" align="center" | height="15" | Probes | ppMALTA1 | ppMALTA1 |- style="font-size:12pt;font-weight:bold" align="center" | height="15" | Sample | Jurkat_gDNA | -ve control |- style="font-size:12pt" align="center" | height="15" | Components | 1x rxn | 1x rxn |- style="font-size:12pt" align="center" | height="15" | Captured Template | align="center" | 20 | align="center" | 20 |- style="font-size:12pt" align="center" | height="15" | 10uM ISB_CA_AF | align="center" | 1 | align="center" | 1 |- style="font-size:12pt" align="center" | height="15" | 10uM ISB_CA_AR_T2 | align="center" | 1 | align="center" | 1 |- style="font-size:12pt" align="center" | height="15" | 2X KAPA SYBR MM | align="center" | 25 | align="center" | 25 |- style="font-size:12pt" align="center" | height="15" | H2O | align="center" | 3 | align="center" | 3 |} Program (Eppendorf Realplex stored under Alan/ampF6.4_template) 98C 30s -> (98C 10s -> 58C 20s -> 72C 20s)x8 -> (98C 10s -> 72C 20s)x15 -> 72C 3min [[File:01212014_qpcr_1.JPG|600px]] *After the 8+15 cycle only GP1_V6 captured seq library got amplified *After meeting with Dr. Zhang, he suggests adding more cycles to the remaining 3 captured product [[File:01212014_qpcr.JPG|600px]] *Amplification happened immediately for all 3 captures, that includes the negative control, which makes me think it is unspecific amplification. I will run a PAGE gel to verify the amplicon size *GP1_V6 amplicon Stored in Maytag -20C (Alan Misc. #20) *illumina_155 amplicon Stored in Maytag -20C (Alan Misc. #21, removed for gel size selection) *ppMALTA1_Jurkat amplicon Stored in Maytag -20C (Alan Misc. #22) *ppMALTA1_H2O amplicon Stored in Maytag -20C (Alan Misc. #23)
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