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==Qiaquick Re-purification== *Add 6x volume of PB buffer to each tube (900ul to 150 ul of DNA) *Transfer 700 ul of the mixture to each QIAquick column (6 columns total, 3 for each set) *Spin at 14,000 rpm for 1 min. Discard the spnt. *Add 750 ul of PE buffer to each tube. *Spin at 14,000 rpm for 1 min. Discard spnt (repeat washing step with buffer PE) *Spin the empty column at 14,000 rpm for 1 min. *Transfer the column to a fresh 1.5 mL tube. *Add 50 ul of 55C EB buffer to each tube *Elute the DNA by spinning at 14,000 rpm for 1 min. *Pool the purified amplicon into the same tube (total volume ~100 ul for each set). *Measure DNA concentration by Nanadrop and calculate the total yield (ds-DNA).
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