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==Combining into One Order== *In GradZhangLab\TB12k_Oct2016 perl padlock2oligo_order.pl > CA12k_Oct2016_Oligos2Order.txt *Total # of probes = 11,154 so copied LungCancer_V8 probes 8X plus a little remainder to bring up to 12k probes -> CA12k_Oct2016_Oligos2Order_FINAL.txt *Double checked probes for all 4 sets **Made sure they were 0 gap **Made sure they were reverse complementary to mRNA **Made sure barcodes were same as decoding probes (so that rolony would be reverse complementary to decoding probes) {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="font-size:11pt" valign="bottom" | width="51" height="14" | Probeset | width="51" | Amplification | width="51" | # Probes | width="51" | # Barcodes | width="51" | # Genes |- style="font-size:11pt" valign="bottom" | height="14" | Human Brain | V4 | align="right" | 4,978 | align="right" | 391 | align="right" | 368 |- style="font-size:11pt" valign="bottom" | height="14" | Mouse Brain | V7 | align="right" | 3,602 | align="right" | 342 | align="right" | 319 |- style="font-size:11pt" valign="bottom" | height="14" | Mouse Embryo | V6 | align="right" | 2,469 | align="right" | 251 | align="right" | 220 |- style="font-size:11pt" valign="bottom" | height="14" | Lung Cancer | V8 | align="right" | 105 | align="right" | 105 | align="right" | 85 |}
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