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=== Step 2. Preparing the Capture Beads === * Step below for 2 captures # Allow the capture beads to warm to RT for 30min prior to use. # Mix the beads thoroughly by vortexing to 15s. # Aliquot 100ul of beads for each capture into a 1.5mL tube. For 2 captures, aliquot 200ul. of beads. # Place the tube with beads on magnet device for 1.5mL tube for 3-5min until the liquid is clear. Remove and discard the liquid. # Add 400ul of Bead Wash Buffer (200ul/sample) # Vortex the beads for 10s # Place the tube back to the magnet. Remove the liquid once the liquid is clear. # Repeat step 2.5-2.7 for a total 2 washes # Resuspend the bead with 200ul Bead Wash Buffer and vortex (100ul/sample) # Aliquot 100ul of resuspended beads into new lo-bind 0.2mL tube. # Place the tube on the magnet. Remove the clear liquid. # The capture beads are now READY to bind to captured DNA. '''PROCEED IMMEDIATELY TO STEP 3. BINDING DNA TO THE CAPTURE BEADS''' <br>
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