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==Objective== *Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA starting amount) with GM12878 gDNA. The challenge of this experiment will be the sample lost due to purification steps and bisulfite conversion step. Qiagen claims 90% recovery rate for the purification columns while Zymo claims a 75% recovery rate for the bisulfite conversion. Resulting in ~360pg of sample library before the library enrichment. Since the recovery rate for the bisulfite conversion is low we will be adding lambda phage dna to the sample to "share" the lost and hopefully will minimize the lose of sample.
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