Editing
AlanFung:LabNotes/Capturing/2014-1-23
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==TBU GEL to verify probe template size== *Aim for 100ng/band *10bp Ladder *ppMALAT1 (92nt, 200uM=6072ng/ul) *MALAT1 Template (45nt, 100uM=1485ng/ul) *ppillumina_155 (99nt, 100uM=3267ng/ul) {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> |- align="center" | width="274" height="13" | Sample | width="75" | ng/ul |style="background-color:#EBF1DE" width="75" | ul | width="273" | ng/ul | width="147" | ul |style="background-color:#EBF1DE" width="75" | H2O |- align="center" | height="13" | ppMALAT1 | align="center" | 6072 |style="background-color:#EBF1DE" align="center" | 0.40 | align="center" | 20 | align="center" | 120 |style="background-color:#EBF1DE" align="center" | 119.60 |- align="center" | height="13" | MALAT1 Template | align="center" | 1485 |style="background-color:#EBF1DE" align="center" | 0.40 | align="center" | 20 | align="center" | 30 |style="background-color:#EBF1DE" align="center" | 29.6 |- align="center" | height="13" | ppillumina_155 | align="center" | 3267 |style="background-color:#EBF1DE" align="center" | 0.40 | align="center" | 20 | align="center" | 65 |style="background-color:#EBF1DE" align="center" | 64.6 |- align="center" | height="13" | 10BP | align="center" | 1000 |style="background-color:#EBF1DE" align="center" | 0.40 | align="center" | 20 | align="center" | 20 |style="background-color:#EBF1DE" align="center" | 19.6 |} *Pre-run the gel for 30 min at 200V *Dilute sample to 20ng/uL *Denature samples and ladder at 75C for 7 min, then quickly transfer to cooling rack, wait for 5 mins *Flush the wells very well with P1000 & P200 pipette to remove all urea. *NOTE: DO NOT TOUCH THE TUBE WITH YOUR HANDS IN THE FOLLOWING STEP, GRAB THE CAP *Load the samples into the wells, run at 200V for 30 min. *Stain gel with 3uL SYBR Gold in ~100 mL of TBE in clean tray. *Place tray on an orbital shaker for ~5 min. [[File:ZhangLab_2 2014-01-23 13hr 49min.jpg|400px]] *1-ppMALAT1, 2-MALAT1_template, 3-illumina_155pp
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information