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==Sample Preparation== *Get GM12878 samples from Noi Took 20ul (250ng/ul) from E2 position of the sample plate, add 30ul ddh2o making final volume to 50ul for shearing *Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o) *Covaris shearing of 5ug gDNA to ~400bp *Contacted Yang at Kelly Frazer's lab to help me with it (so Yang says they don't have the coavris shearing machine anymore and they shear their samples at Biogem now, emailed James and see if i can get it sheared over there) *James helped me out to shear the samples, he used the following settings Target BP 400 Tube Micro Duty Factor 10% Peak Incident Power (w) 140 Cycle per Burst 200 Time (secs) 55 Volume 50ul Temperature (c) 7 Intensifier Yes S water level 12 E water level 6
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