Editing
AlanFung:Protocol/Genome Analyzer
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==SCS v2.4== *start up scsv2.4 ===setting the run parameters=== *click <view> *clear box next to show on start up *from the data collection screen *edit|run parameters|GA Settings / RunBrowser Settings ===GA Setting=== *specify the network location for run results *set perferences for real time analysis results *set settings for auto calibration *delete images (select this option to delete images from the genome analyzer computer after analysis ====Validation strings==== *edit if you are using a customized identification system ====Use auto calibration==== *select this option to enable the auto calibration feature *refer to page 29 for a description of the auto calibration feature *note: if this option is cleared, focus calibration must be performed manually as described in appendix B * ===Run Browser Settings=== *runbrowzer reports, specify which runbrowser reports you want to show and export if selected the first cycle is generated by the software *enable runbrowser report emails to sens reports to the email addresses specified *after you have finished selecting the settings in the run parameters window, click <save & continue>/<cancel & continue>/<reset default configs> ===initialize the software=== *click the manual control/setup tab *perform any operation to trigger the genome analyzer initialization ===run window=== ====recipe tab==== *hover over a stop to show the parameters specific to that step ====image cycletab==== white tile are queued for imaging blue tiles haven been imaged gray tiles are not defined in the current run right side of the window shows the photographs being taken of the current tile ====temperature tab==== *for monitor various run temperatures ===manual control/setup window=== ====manual control==== *settings used for manual focus calibration ====pump tab==== command to solution volume(ml) aspirate rate(ml/min) dispense rate(ml/min) ====recipe viewer==== *genome analyzer recipes found can be found in <install directory>\datacollection_v<#>\bin\recipes *to access the recipe view click file|open recipe from the toolbar *the following information is listed for each recipe recipe subfolder cycle read end multiplexing service version comment file status ====editing cycle number==== *click edit on the recipe viewer toolbar ===reagent tracking=== *provides email notifications and warnings when reagents are getting low and need replacing *beneficial when performing runs longer than 36 cycles *click reagents from the toolbar at the top of the data collection software screen ====current volumes==== ====reset volumes==== ====reagent barcodes==== *to track reagents for any given run barcodes are stored in the Reagentsld.xml file in the runfolder *if the remaining reagents are not to be used click clear ====configure tracking==== *to enable or disable reagent tracking features select reagents|configure tacking enable volume tracking autostop when empty display and email warnings ===disk space checking=== *during the run at the end of each cycle the software checks available disk space *if there is insufficient disk space to accommodate one cycle the software pauses the run and a dialog box appears to tell you how much dish space remains. when sufficient disk space is available the dialog box closes and the run automatically resumes ===image control=== zoom in move the zoomed image zoom out modify the color display view or hide the center mark see intensity values see focus quality and uniformity show region of interest save the image
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information