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===Sample tracking=== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Sample #''' | align="center" style="background:#f0f0f0;"|'''Sample description''' | align="center" style="background:#f0f0f0;"|'''Array''' | align="center" style="background:#f0f0f0;"|'''Image pos.''' | align="center" style="background:#f0f0f0;"|'''Index #''' | align="center" style="background:#f0f0f0;"|'''Barcode''' |- | 1||NeuN+ Cbl 7-03 sorted May 20||1||1||1||ATCACG |- | 2||NeuN+ Cbl 7-03 sorted May 21||1||2||2||CGATGT |- | 3||NeuN+ Cbl 7-03 sorted May 22||2||3||4||TGACCA |- | 4||NeuN+ Cbl 7-03 sorted May 23||2||5||5||ACAGTG |- | 5||NeuN+ Cbl 7-03 sorted May 24||3||6||6||GCCAAT |- | 6||NeuN+ Cbl 7-03 sorted May 25||3||7||7||CAGATC |- | 7||NeuN+ Cbl 7-03 sorted May 26||3||7||10||TAGCTT |- | 8||NeuN+ Cbl 7-03 sorted May 27||4||8||8||ACTTGA |- | 9||NTC from loaded array||4||8||11||GGCTAC |- | 10||NeuN+ Cbl 25-00 sorted May 27||5||10||12||CTTGTA |- | 11||NeuN+ Cbl 25-00 sorted May 28||5||10||13||CCATGA |- | 12||NeuN+ Cbl 25-00 sorted May 29||5||11||14||AAGACA |- | 13||NeuN+ Cbl 25-00 sorted May 30||5||11||15||CTTCCT |- | 14||NeuN+ Cbl 25-00 sorted May 31||6||12||25||TACTGC |- | 15||NeuN+ Cbl 25-00 sorted May 32||6||12||26||TCGTGA |- | 16||NeuN+ Cbl 25-00 sorted May 33||6||13||16||GGGGTT |- | 17||NeuN+ Cbl 25-00 sorted May 34||6||13||27||TACGCG |- | 18||NeuN+ Cbl 25-00 sorted May 35||7||14||28||AGGTGC |- | 19||NeuN+ Cbl 25-00 sorted May 36||7||14||29||ACGAAC |- | 20||NeuN+ Cbl 25-00 sorted May 37||7||15||30||TTAGTG |- | 21||NeuN+ Cbl 25-00 sorted May 38||8||16||31||CACCAC |- | 22||NeuN+ Cbl 25-00 sorted May 39||8||16||33||CGCAAC |- | 23||NeuN+ Cbl 25-00 sorted May 40||8||17||34||AACTGA |- | 24||NTC from loaded array||8||17||35||ATCGGG |- | |} * I used a different type of array, the last one from the Groisman lab. * It contained a type of bulk blocking treatment that would wet and block PDMS pores without BSA. * They told me to use it without BSA, but I used BSA anyways. * I saw a lot of wrinkling in the bottom of the array due to its thinness. This created a lot of issues with imaging and picking.
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