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===Before Starting Protocol=== 1. Check if have enough reagents etc for the protocol *lysis buffer *T7-top2 transposomes *custom nextera transposomes *transposase/transposome *IVT reaction mixture *cells etc *Rnase H, random hexamers, protease, AMPURE beads ,ATP, taq polymerase, other polymerases (BST, klewnow exo-, DNAPI), other primers *2. samples this time, 200 ng each sample if not stated. Samples: Index 60, NXTA adaptor 1. PBS 1K cells Index 61, NXTA adaptor 2. PBS 12 uL 1000 cells Index 62, NXTA adaptor 3. FACS buffer 1K cells Index 63, NXTA adaptor 4. FACS buffer 1K cells (FORGOT TO ADD: 5 mM MgCl2) Index 64, NXTA adaptor 5. FACS buffer 12 uL 1000 cells Index 65, NXTA adaptor 6. 6 ng pure Jurkat DNA Index 66, NXTA adaptor 7. 1.- PBS 1K cells, Zymo DNA purified Index 67, NXTA adaptor 8. 3.- FACS bf 1K cells Zymo DNA purified Index 68, NXTA adaptor 9. 4.- FACS bf 1K cells 800 ng RNA Index 69, NXTA adaptor 10. 4.- FACS bf 1K cells 400 ng RNA Index 70, NXTA adaptor 11. 4.- FACS bf 1K cells 100 ng RNA Index 71, NXTA adaptor 12. 4.- FACS bf 1K cells 50 ng RNA Index 72, NXTA adaptor 13. 1000 cells lysed w/out tspn
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