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===Before starting protocols=== 1. Check if have enough reagents etc for the protocol *lysis buffer *sc1 transposomes *custom 3' transpson *IVT reagents *cells etc *5X, 2X taq polymerase *Zymo DNA clean and concentrator *2. Samples *pool samples 1-30. 1. sc1-T7iBR-Idx25 puf., 10 cells 2. sc1-T7iBR-Idx26 puf., 10 cells 3. sc1-T7iBR-Idx27 puf., 10 cells 4. sc1-T7iBR-Idx28 puf., 10 cells 5. sc1-T7iBR-Idx29 puf., 10 cells 6. sc1-T7iBR-Idx30 puf., 10 cells 7. sc1-T7iBR-Idx31 puf., 10 cells 8. sc1-T7iBR-Idx32 puf., 10 cells 9. sc1-T7iBR-Idx33 puf., 10 cells 10. sc1-T7iBR-Idx34 puf., 10 cells 11. sc1-T7iBR-Idx35 puf., 10 cells 12. sc1-T7iBR-Idx36 puf., 10 cells 13. sc1-T7iBR-Idx37 puf., 10 cells 14. sc1-T7iBR-Idx38 puf., 10 cells 15. sc1-T7iBR-Idx39 puf., 10 cells 16. sc1-T7iBR-Idx40 puf., 10 cells 17. sc1-T7iBR-Idx41 puf., 10 cells 18. sc1-T7iBR-Idx42 puf., 10 cells 19. sc1-T7iBR-Idx43 puf., 10 cells 20. sc1-T7iBR-Idx44 puf., 10 cells 21. sc1-T7iBR-Idx45 puf., 10 cells 22. sc1-T7iBR-Idx46 puf., 10 cells 23. sc1-T7iBR-Idx47 puf., 10 cells 24. sc1-T7iBR-Idx48 puf., 10 cells 25. sc1-T7iBR-Idx49 puf., 10 cells 26. sc1-T7iBR-Idx50 puf., 10 cells 27. sc1-T7iBR-Idx51 puf., 10 cells 28. sc1-T7iBR-Idx52 puf., 10 cells 29. sc1-T7iBR-Idx53 puf., 10 cells 30. sc1-T7iBR-Idx54 puf., 10 cells 31. sc1-T7iBR-Idx55 puf., 500 cells 32. sc1-T7iBR-Idx56 puf., FACS NTC (sheath buffer run thru facs) 33. sc1-T7iBR-Idx25 puf. diluted 2X ~ .5 uL tspsome added, 3 ng Jurkat DNA 34. sc1-T7iBR-Idx26 puf. diluted 2X ~ 1 uL tspsome added, 1000 cells
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