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===Before starting protocols=== 1. Check if have enough reagents etc for the protocol *2. Samples *transpoase was either directly annealed to transposon, diluted in storage buffer then annealed to transposon, diluted 2X more after being diluted in storage buffer, or 4X more after being diluted in storage buffer. samples (all use buffer D6) 1. T7tspn-top2, E1 direct anneal (4.22 uM), 6 ng DNA 2. T7tspn-top2, E1 strg-anneal (1.77 uM), 6 ng DNA 3. T7tspn-top2, E1 2X dil. strg-anneal (.88 uM), 6 ng DNA 4. T7tspn-top2, E1 4X dil. strg-anneal (.44 uM), 6 ng DNA 5. T7tspn-top2, E2 direct anneal (3.6 uM), 6 ng DNA 6. T7tspn-top2, nxta direct anneal, 6 ng DNA 7. T7tspn-top2, nxta strg-anneal, 6 ng DNA 8. T7tspn-top2, nxta 2X dil. strg-anneal, 6 ng DNA 9. T7tspn-top2, nxta 4X dil. strg-anneal, 6 ng DNA 10. pure DNA only, 6 ng DNA 11. NTC
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