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===Validation of THS-seq barcoded transposons using Nextera XT v2 sequences for multiplex combinatorial indexing=== *designed scT7_r5XXX_i5_top transposon so it can be used used with 6 base pair barcodes. 96 barcoded transposons made. Will first test with tn5059 transposomes from Trina for validation the transposon is working and that fragments can be sequenced on MiSeq/HiSeq. Then will proceed to order the rest of the transposons and use with combinatorial sequencing with THS-seq for single cells. *There is slight modification to MiSeq protocol, custom primers will need to be used and Read2 will instead be a 6 basepair index reading using r5_index_read. Standard Nextera XT v2 oligos i5 index, i7 index and read1 are used for sequencing.
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