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===lysis buffer 1=== *Crawford et al. PNAS 2003 *lysis buffer composition: 10X lysis buffer 100mM Tris.Hcl pH 7.5 100mM NaCl 30mM MgCl2 1% IGEPAL® CA-630 (replaces NP40) *make 1M solution of NaCl and MgCl2 1M NaCl, 1.461 grams in nuclease free/DI water and bring to 25 mL 1M MgCl2, 2.3805 grams in nuclease free/DI water and bring to 25 mL (add mgcl2 to water, very exothermic!!) *To make 10 mL of lysis buffer 500 uL of 2M Tris.Hcl PH7.5 1000 uL of 1M NaCl 333 uL of 1M MgCl2 100 uL of IGEPAL® CA-630 (1% in soln, replaces NP40) 8.067 mL Nuclease free water {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''10X''' | align="center" style="background:#f0f0f0;"|'''100X''' | align="center" style="background:#f0f0f0;"|'''50X''' | align="center" style="background:#f0f0f0;"|'''40X''' | align="center" style="background:#f0f0f0;"|'''''' |- | Tris-HCl pH 7.5||2.0M||500||5000||2500||2000||uL |- | NaCl||5M||200||2000||1000||800||uL |- | MgCl||1M||333||3330||1665||1332||uL |- | IGEPAL CA-630||solid||100||1000||500||400||uL |- | ||||||0||0||0||uL |- | |||||||||||| |- | |||||||||||| |- | ||||||11330||5665||4532||uL |- | ||||H20||-1330||4335||5468||uL |- | |}
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