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==Background== *Over the past few weeks, I've made ~1ug of padlock probes using Noi's probe production protocol (details to be posted soon) *Today, we want to test these probes (designed 5/12) on capture efficiency of human genomic DNA provided by Alan (GM12878 P21 [5/23/14] 148ng/ul) *The overall experimental design today will be testing the target capture using three probe:target ratios and three human gDNA input amounts **Probe:Target ratio = 200:1, 300:1, 400:1 ***This was based on Noi's recommendation that we may need to use a higher probe:target ratio because only have ~9000 unique probes designed in the oligo mix **Target input amount = 50ng, 100ng, 200ng ***This is in order to obtain a comparable result to Fluidigm's C1 output of ~100ng of MDA product *In the future, if this experiment works, this will optimize most the Probe:Target ratio necessary for successful capture so we will be able to move on to capture of in-tube MDA amplicons
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