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==Background== *So far, I have not gotten a good probe capture result yet (as the result of capture is usually a small band while mine is currently still a smear on the gel after PCR) *One of the suggestions given in the previous lab meeting was the increase the number of controls during each run of the experiments. Because of this, I'll be adding a new negative control to be added in after 20hr annealing but before Hemo KlenTaq gap filling and circularization. I'll also be adding in a reaction for Rui's probe as a positive control **Note that for Rui's probes, I'll need to do a 60C annealing. Also, Rui uses 3.2ul of 0.1ng/ul (need to dilute 1/10 from the 1ng/ul aliquot that she gave me) probe for 20ng of gDNA. So, for my experiment, will need to add 16ul of 0.1ng/ul probe for 100ng gDNA. This amounts to a 1000:1 probe:target ratio. *Also will try a condition with lower probe:target ratio, as this may be a possibility for the high intensity of the non-specific band.
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