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==Background== *This is a continuation of the experiment started on <http://genome-tech.ucsd.edu/LabNotes/index.php/Chris:LabNotes/FateMapping/Calendar/2015/2015-3-12> *As a reminder, the following are the reactions for this experiment: ---------------------------Extended MDA Hemo KlenTaq (Strip A)------------------------------------ rxn Target (ng) Probe:Target Probe (ng) Description 1A 50ng (MDA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA MDA Control (Hemo KlenTaq) 2A 50ng (gDNA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA Control (Hemo KlenTaq) 3A 50ng (Extended G1) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well G1 (Hemo KlenTaq) 4A 50ng (Extended H1) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well H1 (Hemo KlenTaq) 5A 50ng (Extended A2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well A2 (Hemo KlenTaq) 6A 50ng (Extended B2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well B2 (Hemo KlenTaq) 7A 50ng (Extended C2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well C2 (Hemo KlenTaq) 8A 0ng (Extended NTC) -- 3.69 (CW 9/22) CW 9/22 Extended NTC (Hemo KlenTaq) <- NTC from the extension MDA reaction --------------------------------Extended MDA Phusion (Strip B)------------------------------------ rxn Target (ng) Probe:Target Probe (ng) Description 1B 50ng (MDA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA MDA Control (Phusion) 2B 50ng (gDNA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA Control (Phusion) 3B 50ng (Extended G1) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well G1 (Phusion) 4B 50ng (Extended H1) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well H1 (Phusion) 5B 50ng (Extended A2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well A2 (Phusion) 6B 50ng (Extended B2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well B2 (Phusion) 7B 50ng (Extended C2) 500:1 3.69 (CW 9/22) CW 9/22 Extended C1 Well C2 (Phusion) 8B 0ng (Extended NTC) -- 3.69 (CW 9/22) CW 9/22 Extended NTC (Phusion) ---------------------------------C1 Hemo KlenTaq (Strip C)---------------------------------------- rxn Target (ng) Probe:Target Probe (ng) Description 1C 50ng (MDA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA MDA Control (Hemo KlenTaq) 2C 50ng (gDNA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA Control (Hemo KlenTaq) 3C 50ng (C1 Well G1) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well G1 (Hemo KlenTaq) 4C 50ng (C1 Well H1) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well H1 (Hemo KlenTaq) 5C 50ng (C1 Well A2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well A2 (Hemo KlenTaq) 6C 50ng (C1 Well B2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well B2 (Hemo KlenTaq) 7C 50ng (C1 Well C2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well C2 (Hemo KlenTaq) 8C 0ng (NTC) -- 3.69 (CW 9/22) CW 9/22 NTC (Hemo KlenTaq) <- This is just water added into the reaction ------------------------------------C1 Phusion (Strip D)------------------------------------------ rxn Target (ng) Probe:Target Probe (ng) Description 1D 50ng (MDA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA MDA Control (Phusion) 2D 50ng (gDNA) 500:1 3.69 (CW 9/22) CW 9/22 gDNA Control (Phusion) 3D 50ng (C1 Well G1) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well G1 (Phusion) 4D 50ng (C1 Well H1) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well H1 (Phusion) 5D 50ng (C1 Well A2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well A2 (Phusion) 6D 50ng (C1 Well B2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well B2 (Phusion) 7D 50ng (C1 Well C2) 500:1 3.69 (CW 9/22) CW 9/22 C1 Well C2 (Phusion) 8D 0ng (NTC) -- 3.69 (CW 9/22) CW 9/22 NTC (Phusion)
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