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==Background== *Previously, I tried ETOH precipitation with tRNA carriers in order to concentrate the sample to fit into the 5ul Tn5 reaction (see <http://genome-tech.ucsd.edu/LabNotes/index.php/Chris:LabNotes/SeaMicrobiome/Calendar/2015/2015-4-10>). However, that experiment failed. *In order to get around ETOH precipitation, we will try to do another test in which we increase the volume of the Tn5 reaction to account for the most dilute samples (38pg/ul in 11ul). In order to do this, we will increase the reaction volume to 15ul (instead of 5ul) and use only 10ul of original sample *Below is a summary of the reactions for this experiment: rxn Sample Template Input Enzyme Vol (0.25x conc) 1 UO2-NP 10ul 38pg/ul 1ul 2 UO3-NP 10ul 38pg/ul 1ul ------------------------------------------------------------- 3 UO2-NP 10ul 38pg/ul 2ul 4 UO3-NP 10ul 38pg/ul 2ul ------------------------------------------------------------- 5 UO2-NP 1ul 1ng/ul 1ul 6 UO3-NP 1ul 1ng/ul 1ul ------------------------------------------------------------- 7 UO2-NP 1ul 1ng/ul 2ul 8 UO3-NP 1ul 1ng/ul 2ul ------------------------------------------------------------- 9 NTC -- 1ul
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