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===T4 BGT glucosylation === * Set up (150 uL total volume): unsheared gDNA 17.94 uL (2.1 ug) 50X UDP-glucose 3.00 uL 10X NEB Buffer 4 15.00 uL Nuclease free H2O 109.77 uL T4 BGT 3.00 uL 5hmC/5mC/C controls 1.29 uL * Incubate at 37C for 29 hours. * Purify with 1.8X AMPure beads. * Elute in 40 uL water total. DO not elute in EDTA, DNA should be free of EDTA. * Qubit: 27.9 ng/uL x 40 uL = 1116 ng (53% yield) * Leave DNA on beads!
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