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===Exp.2: Repeat Exp.1 on the three PGP1 cDNAs using NEB's 2X Taq Master mix=== Set up reactions <b>on ice</b>: One 33x Template 1ul 33ul 2X Taq Mix 25ul 825ul 50X SYBG I 0.4ul 13.2ul 10uM Primers 1ul each H2O 22.6ul 746ul 94C 2min -> 40 cycles of (94C 30S -> 56C 30S -> 68C 1min) -> 68C 3min on Chromo4. ====Result==== Few reactions with ThermoStart Taq worked. Taq 2x mix is a lot better, the curves for most amplification came up. I had some issues with running agarose gel. I check A1-A10 on 8% PAGE gel, and they look good. I will repeat the amplification on GM20431 gDNA, Hues6 gDNA, and Hues6 cDNA.
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