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==Exp.2 Perform QPCR to test whether the PGP1 cDNAs contain gDNA contaminations== To estimate the level of gDNA contaminations, I use two sets of six PCR primer pairs (A1,B1,C1,F1,G1,H1) in the PGP1VER2 plates, one set targeting cDNA and the other targeting gDNA. GM20431(06/05) GC1F(06/05) GC1EP(06/08) Hues6(05/22) x 50 Template(2ng/ul) 1ul 1ul 1ul 1ul 12 x 4 2X Taq Master mix 15ul 15ul 15ul 15ul 750 2uM primer mix 5ul 5ul 5ul 5ul each 50X SYBG I 0.2ul 0.2ul 0.2ul 0.2ul 10 H2O 8.8ul 8.8ul 8.8ul 8.8ul 440 94C 2min -> (94C 30sec -> 57C 30sec -> 72C 45sec)x 40 -> 72C 3min -> 15C hold [[Image:2007-12-31-gDNA-contamination-check.png|400px]] <b> I think all the PGP1 RNA samples (GM20431, GC1F, GC1EP) have various level of gDNA contamination. Hues6 seem to be cleaner.</b>
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