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===Padlock capture=== *Probe sets: **Jan09-1: 46,417 probes, 334nM **Jan09-2: 53,493 probes, 294nM **Jan09-3: 53,828 probes, 186nM *Template DNA: PGP1, sheared with Bioruptor, 50ng/ul; 200ug per reaction. *Probe:target ratio: 200:1 Shaking Control Template(50ng/ul) 4ul 4ul 10X AmpLigase buffer 2ul 2ul Jan09-1 (334nM) 3.1ul 3.1ul Jan09-2 (294nM) 4.0ul 4.0ul Jan09-3(unknown) 4.0ul 4.0ul H2O 2.0ul 2.0ul 95C 2min -> -0.02C/sec to 60C -> (move one tube to ThermoMixer @60C, 400rpm) ->60C 18h -> add 1ul SLN mix (dNTP 5uM, Stoffel 2U/ul, AmpLigase 1U/ul) -> 60C 24h -> 94C 1min -> 37c 1min -> add 2ul Exo I&III -> 37C 2h -> 90C 5min -> 4C hold
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