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====RT RAB7A Results==== *Previously Hosuk (using FISSEQ_Adpt dye probe)found that primers of different distances from RAB7A TSS produced different numbers of primary rolonies **Increasing distance from TSS correlated with increasing # of primary rolonies with 1000bp having even more rolonies than produced by random hexamer primer **However using a dye probe specific for RAB7A target (RAB7A_1stRolonyFISH) which should be on all RAB7A primary rolonies since it is within 100bp of TSS no signal was found ***This is why experiment is worth repeating Checked here: [[Matt:LabNotes/2014-1-28]]
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