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==Conclusion== *[https://www.lifetechnologies.com/order/catalog/product/A20004 Alexa594] is significantly larger, more hydrophobic, and less charged than [https://www.lifetechnologies.com/order/catalog/product/A20000 Alexa 488] **Therefore possible size exclusion column is not as effective at removing it **And more likely to precipitate? **Try using PCR purification column instead of Centri-Sep column *Use Alexa647 instead (with Alexa488) **Dr. Bern's microscope does not have far red filter **Must use Dr. Wang's Nikon II microscope (doesn't have DAPI) **See if Dr. Huang's lab has FITC, Cy5, and DAPI filters with >60X Obj and 1.4NA and <-20C CCD
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