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==Link PKP2_control oligo to Streptavidin Bead== #Make 2X B&W (Binding and Wash) Buffer #*16ml 5uM NaCl + 80ul 5uM EDTA + 100ul 4uM Tris-HCl + 23.82ml H2O #Resuspend beads by vortexing #Transfer 100ul of beads (10ug/ul) to new tube #Pull down by magnet 2min and remove supernatant #Wash 3x with 100ul 1X B&W Buffer by resuspending and then removing supernatant #Resuspend in 200ul 2X B&W Buffer #Add 200ul of 2.5uM PKP2_Control #Incubate 15min at RT gently rotating #Pull down with magnet #Wash 3x with 1X B&W Buffer #Resuspend in 1ml 1X PBS
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