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==Calculate molar concentrations in final mix== *5% Acrylamide = 700mM **5g/100mL **71.08 g/mol *0.1% APS = 4.4mM **100mg/100ml **228.18 g/mol *0.1% TEMED = 6.67mM **2ul/2ml **116.24 g/mol **775 kg/m^3 = 775 g/L *Pick concentration for BS(PEG)9: *BS(PEG)9 = 5mM **708.71 g/mol **After adding 465ul DMSO to stock bottle: 100mg/565ul = 177 g/L = 250mM **Dilute 50X in final mix just like APS and TEMED: 5mM *Pick concentration for Acrydite/Amine Oligo Linker **Ideally ~1mM in final mix but due to only having 61.1 nmol in tube have to use less **Resuspend 61.1 nmol in 123.2 ul = 500uM **Dilute 2X in final mix so final concentration: 250uM
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