Editing
Matt:LabNotes/2017-6-27
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Prepare Beads== #Prepare 50ml Dynabeads buffer: 20ml 5M NaCl + 100ul 0.5M EDTA + 125ul 4M Tris-HCl + 29.775ml H2O #*2M NaCl, 1mM EDTA, 10mM Tris-HCl #Do 2 tubes, 1 for BarcodeV1 and 1 for BarcodeV3: #Add 1ul Dynabeads MyOne Streptavidin C1 beads to 100ul buffer #Vortex #Pull down 1min on magnet #Remove supernatant #Combined 15ul buffer with 15ul amplicon #*BarcUv1 500nM (dsDNA PCR product with Uracil prepared by Dan 5.25.2017) #*BarcUv3 500nM (dsDNA PCR product with Uracil prepared by Dan 5.25.2017) #Add to beads, pipette mix, and incubate 15min at room temp #Pull down #Wash twice with 100ul buffer (mix off magnet, and then pull down on magnet) #Bring volume to 40ul with H2O
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information