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==Purpose and General Procedure== *Make gel to cover and mechanically stabilize brain tissue section for DARTFISH to prevent tissue degradation *Instead of using dessicated bind-silane treated slides we have to use vectabond-treated cover glass in a tissue culture dish with a hydrated tissue section **Due to thickness of culture dish (~1mm) no way to use coverslip to prevent exposure to air while making <1mm thick gel ***Can try using mineral oil to cover instead *Not using DATD (cross-linker) in Acrylamide/Bis mix
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