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== Sanger sequencing results of the amplicons amplified with randomly tagging primers (with USER)== '''AmpF7AUSol:''' AATGATACGGCGACCACCGAGAUCUACAC'''AAAAAAA'''CACUCUCAGAUGTUAUCGAGGUCCGAC<br> '''AmpF7AUSol:''' AATGATACGGCGACCACCGAGAUCUACAC'''NNNNNNN'''CACUCUCAGAUGTUAUCGAGGUCCGAC<br> '''Syb_FP5A:''' AATGATACGGCGACCACCGAG<br> '''Syb_RP7:''' CAAGCAGAAGACGGCATACGAG<br> '''Check Sanger's sequencing result roughly before sending more clones''' * Primimg with Syb_RP-7: read reverse complementary of the AmpFA/NU.Sol strand '''qs (quality score)''' '''Sequence correct''' '''7nt=AAAAAAA''' '''Note''' '''contain AGAGTG(7A or7N)GTG''' 1U-1 29 seem to (overlapping peaks) yes, :Homopolymeric or Repetitive Region, **request for free repeat 1U-2 29 yes yes, :Homopolymeric or Repetitive Region 1U-3 33 yes yes, chromatogram very clear even the peak very low 1U-4 32 yes yes, 1U-5 42 yes yes, 1U-6 43 yes yes, 1U-7 42 yes yes, chromatogram very clear even the peak very low 1U-8 14 seem to (overlapping peaks) yes Non-specific, **request for free repeat 1U-9 31 yes yes, 1U-10 43 no no, This clone has a shift band higher than other positive clones on E-gel will look closer to the sequences (seem to be neither AmpFNU.Sol nor AmpFAU.Sol)
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