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== Sample transfer from 384-well plate to 96-well plate == '''Sample vial#1 (AC-718)''' * There are total 13 samples will be transferred from 384-well plate to 96-well plate: '''Labeling: AC-718-96well_plate1''', in the following positions {| {{table}} | align="center" style="background:#f0f0f0;"|'''Position in AC-718-384-well''' | align="center" style="background:#f0f0f0;"|'''Position in AC-718-96-well_plate1''' |- | F03||A1 |- | J03||B1 |- | L02||C1 |- | M05||D1 |- | N03||E1 |- | P02||F1 |- | P04||G1 |- | P05||H1 |- | P07||A2 |- | N17||B2 |- | A21||C2 |- | J04||D2 |- | K06||E2 |} * CSV input file: [[Media:AC-718-384-well-to-AC-718-96-well_plate1.csv| AC-718-384-well-to-AC-718-96-well_plate1.csv]]. Note: I set up a larger sample volume (15ul) than the actual volume to make sure all liquid is transferred to the new plate * The first eight amplicons from plate 718 will be used for rehearsal (AC-718-96-well_plate1, A1-H1). * For AC-718-96-well_plate1, transfer ~2.5ul A1-H1 to A1-H1 96-well shearing plate for Illumina library (400bp), and transfer ~2.5ul A1-H1 to A2-H2 96-well shearing plate for PacBio library (2kb) * Note: When I try pipetting the sample in 384-well plate with epMotion, the total volume is only 8ul. I then will use only 2.5ul for each library prep. To pipette a very small volume from a tiny well, I need to set the speed to be very low (0.4mm/sec) otherwise there will be some liquid left in the well (~1ul). '''Sample vial#2 (AC-777)''' * We will split the amplicons in this plate into 4 96-well plates {| {{table}} | align="center" style="background:#f0f0f0;"|'''Position in AC-777-384-well''' | align="center" style="background:#f0f0f0;"|'''96-well plate labeling''' |- | A1-H12||AC-777_plate1_A1-H12 |- | I1-P12||AC-777_plate2_I1-P12 |- | A13-H24||AC-777_plate3_A13-H24 |- | I13-P24||AC-777_plate4_I13-P24 |}
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