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=== 2013_04_23 === * Even Jeff will process his MDA products using CGI protocol, we still want to try the standard protocol following Rui's condition she used for her experiment. * Things have been adjusted ** DNA shearing: I included the 200bp fragment to compare if the size effects adapter ligation efficiency (Rui: 200bp fragment) ** Prepared the same concentration of adapter (30uM) and used the same concentration for ligation ** Amplification: *** Annealing temperature: I previously used 65C for annealing. I reduced annealing temperature to 62C (Rui: 60C with the same pair of primers). *** Reduced volume from 100ul to 50ul *** Slightly changes were noted during each step * Sample list: I still included higher amount of DNA (2.5 & 5ng) to compare the improvement by adjusting the conditions. ** NTC, 1ng_200bp, 2.5ng_200bp, 5ng_200bp, 1ng_400bp, 2.5ng_400bp, 5ng_400bp
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