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=== Oligo info. === * Name: 90k_oligos_30Sept2014 * Conc. 69.89ng/ul, * Volume 80ul in TE buffer. Total amount 5.59ug * [[Kun:LabNotes/MONOD/2014-8-4#Re-design_of_selector_probes| Info from Dr. Zhang's note]] *Sept14 probe set: [[Media:90k_oligos_30Sept2014.txt.gz]] probe set # probes Length Amp primers Chris gDNA_MS_v2 8,045 127bp V6(G*T*CATATCGGTCACTGTU//5Phos/GGGTAGTGTGTATCCTG) Kun cancer_hyb_sept14 51,639 110-130bp V8(T*C*TAATCTAGCGCGACGTCU//5Phos/CCACAAGAGGCGCTATG) Kun LMS_selector 17,342 124-130bp NE(TGCCTAGGACCGGATCAACT/GCTTCGGTTCACGCAATG) Kun padlock_SNPs 12,974 125bp V4(G*A*CTGGAAGAGCACTGTU//5Phos/AGCCTCATGCGTATCCG) Total 90,000 * Length: I would use the average size of the oligo pool to calculate concentration ~125nt = MW=41250g/mole * Conc. : 69.89 ng/ul = 1694.30 (69.89ng/ul/ 41250g/mole)
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