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Protease and Proteinase Trials
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==3/31/10== ===Setup=== 2 columns/strips of sorted chromosomes were collected from Sam. The strips were separated as follows: | H | - Head Row 1 | o | - No chromosomes Row 2 | o | - 1 chromosome Row 3 | o | - 12 chromosomes Row 4 | o | - 12 chromosomes Row 5 | o | - 12 chromosomes Row 6 | o | - 23 chromosomes Row 7 | o | - 23 chromosomes Row 8 | o | - 23 chromosomes | A | - Tail Protease was added as follows: Rows 1,2,5,8: No P. added Rows 3,6: 1uL P. added Rows 4,7: 0.5uL P. added The same was applied to both strips, labelled Strip 1 and Strip 2 ===Incubation=== Strip 1: 37C, 10min -> 70C, 15min Strip 2: 37C, 20min -> 70C, 15min ===Results=== To conserve resources, only the 23 chromosome wells were imaged. Any effects on 23 chromosome wells were assumed to have had similar effects on the 12 chromosome wells. [[image: 23chr_10min_1uL.jpg]] ''Image 1: 23chr, 10min incubation, 1uL protease added'' [[image: 23chr_20min_1uL.jpg]] ''Image 2: 23chr, 20min incubation, 1uL protease added'' ===Discussion=== Protease had no noticeable effect on the chromosomes. Two likely reasons for this: 1) short incubation time. 2) not enough protease added. Also, had no frame of reference because a positive control was not added into this trial (i.e. 0.5mg/mL Proteinase K)
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