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===RNA sample Hues54=== * 15 colonies directly for TempliPhi, roughly ~500ng/ul in 10ul PCR, 3ul sending for sequencing (M13R provided by Genewiz) * No priming: 3 * mRNA: 11 * Potential non-coding region: 1 RNA-1: poor quality; first 60bp match to intergenic region with 88% >RNA-1-M13R_F01.ab1 NNNNNNANNNTGNNNNNNGCTCGGATCCNCTAGTAACGGCCGCCNGTGTGCTGGAATTCGGCTTTAATACGACTCACNNN NGGNGTATGCTTTTTTTTTTTTTTTTTTTTTTTTTCCNCNGGAANAANTGCACCTGTGTCGCCNTCCCACAGGGGAACTC GGGAGACAAAGGCGCTTTGNCNCNTCTGGGGGANCCTATCAGGACCAATTCCCATGGCGAATACCATGATATCGTCCGTN ACTGGGGGGANANTGACNTGGNTCCNAANACTGNGGNTCGGCCCGATNTTTANAAAAAAACNAACGCTAAANAAAATTGA CAATTCCAAATTAACCGGTTCCCCGTAGANCTTTCGGGGGNGGGGANCNGGGGANNNNNNNGGNCTTTCCNNCNNCCANA ANAAAAAAGAAAGGGACGGGCCGGGGAAAGGCCGGNGAAACAGGCNATAGNNCCTAGGACANTGNNNATGATNAAGACCG ACTAATGGGGGCTGCGGNCCCGTGTGTANGNGTCAGGCTGTTTCTTTTGGNANGAAGAGGGGTGGTCTTTCATANAATTC GGTGGGGGGTGGGCTCGGAGAGGGTCTACTTTGTGGAGAGNATGANGGCCAANNNANNCCNAAAGCCNAGCACNTCGGCG AAGATCAGAATCCGANNTACCNNTN RNA-2: "poor quality"; spliced form of RPL35, 98.1% >RNA-2-M13R_F02.ab1 NNNNNNNNNNNNNNNNNNNNNCTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTGGAGCTGCTGAAACAGCTGGACGACCTGAAGGTGGAGCTGTCCN NNCTGCGCGTCGCCAAAGTGACAGGGGGNGCGGCCTCCAAGCTCTCTAAGATCCGAGTCGTCCGGAAATCCATTGCCCGT GTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGTAGTACAAGCCCCTGGACCT GCGGCCTAAGAAGAAACGTGCCATGCNCCGCCGGCTCAATAANCACGANGANAACCTGAAAACCAAGAAGCAGCAGCGGA AGGAGCGGCTGTACCCGCTGCGGAAATACCCGGTCAAGGNCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGNTGAGAA A RNA-3: "poor quality; spliced form of BQBP1, 98.4% >RNA-3-M13R_F03.ab1 NNNNNNNNNNNNNNNNNNNNNNCTNNNNATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTNNNGGGGGANCCTTTTTTTTTTTTTTTTTTTTTTTTTCCCNNGANANGNTCTGNCAGGGATCGAGAGCGTGGNTATGA CAAGGTAGACAGANAGAGAGAGCGANNGNNNNAACGGGATCGGGACCGCGGGTATGANAAGGCAGACCGGGAANAGGGCA AAGAAGGGCGCCCCCATCGCCGGGAGGAGCNGNCTCCCTATCCCAAGAGCNAGAAGGCAGTAAGCCGAAAGGATGAAGAG TTANACCCCNTGGACCCTAGCTCATACNCNNACGCCCCCCGGGGCNGTNGNCAACAGGANTCCCCAAGNGGAATGAGGCN AAAAN RNA-4: PQ; MTF1, 98.6% >RNA-4-M13R_F04.ab1 NNNNNNNNNNNNNNNNNNNNNTCGGATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACTA NAGGGGNNNCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGCCGACAAGCAGAGACTCCTTCAGACCCTCAAAC AGAAACATTAAGTGCCATGGATGTGTNAGAGTTTCTNTCCCTCCAGAGCCTGGACACCCCGTCCAATCTGATTCCCATTG AAGCACTACTGCAGGGGGAGGAGGAGATGGGCCTCACCAGCAGCTTCTCCAAGTNAAGGGCCCATGTGTGCTCCCCTCTG GGAAAAGCGGGTGAGCAGGAGGCATGAGGTACAATGCCTGCCATCATGGGTCAAAAATTTGAAGGATGAAAAAATCTACT GTTTGAAATCCTCACCTTTTAGACGTATTTTTTTTATTCAN RNA-5: PQ; SNX9, 99.5% >RNA-5-M13R_F05.ab1 NNNNNNNNNNNNNNNNNCNNANNNNNNNNNCACNANTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATANNGNGNGNNTTTTTTTTTTTTTTTTTTTTTTTAACAGGNNCNNGNNACACCGTCAGCTCGAACTCAACACTGGCAG CCACCGTCTCACCCCTGCGGAGGAGCGCTCCCGTCTCCCACAGGTGCCTTACCGCGTTCCCTCCCGCTGCTTTCATTTTT CTGACCTAATAATTACGGGAAANGGAAAGTCTGGGCCANCANCNATAAAATGACACCAAAAATAAGTANANGANATCGAA T RNA-6: no priming RNA-7:PQ; CR594125, 94.6% >RNA-7-M13R_F07.ab1 NNNNNNNNNNNNTGNNNNNAGCTCGNATNNNNTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ANNGGGGNNNCCNTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTCCCCGTGGTTTAATATGAACAGAGTTGAATATGACAT TGTCTGACAAAAGAATGAACAATTTGCTGAATAAAAGCCCCAAGTCAGGATATATACACAGAAGAAATGGAGCCTCAGAG TCTCAGCACTTGTGCNCAATGAAAGGAGAAATCTTTAAAAAAAAAAATCTGTAACAGAGGATAAAAATCAAAAGGCAGCA AGGACACCGACACTGGNGAGGTGGGAAAGGGGCTNNTCANCTACCAGCATGGGCTCNNCNGCTAGAGAGCTAACCNATNT CAAGGATTTTCAACNCTGAGGTGGGTTCCANNTCCCCTGNN RNA-8: spliced NUCKS1, 99.7% >RNA-8-M13R_F08.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNCCNCTAGTNNCGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTCGAACTGCCATAGTCACTATCGTCATCATCTTCCATTAGGAAAT CTTCATCGCTGCCGGAATTCTCCTGGAATGGTGCCTCATCCTCCTCTTCTTGTTCTTCCTCACTGCCCACATCTTCCATG AGCATCTCTCTCTGTTTAGAAGCTGCTTTCGATGCCGCCTGCCGTTGTTGGCGCACATTTTTATGATCTTCTTTTTCATC TTCACTATCCTCTGCTGAGTGAGAATCATCCTTCTTGGTCTTCACATCTTTGTCTTCTGAGTCCTCACTATCTTCCTGTG AATTCTTTCCAGATCGCCTCTTATTTTTAGCTTCTCGGGGAGATGATCGAATTTTCTTAGTGGGAGGGCCCGAATCTCTT CCATAATCTTCATCTGCNTCATCAGATTCCTGAAACTGTGAGTAATCAACAACCTTCCTATTTCTGACAGGCCGCGACAT GTTCGCTGTCGAAACAGGACCGAGTCGAGAAGCCAAAGACCAGGACCCCCCCTACCCCGCGCGCTCGGCGCCCCACCCAA AANNNANNNAAANANAAAAGGGATACCCCTATNNNNNNTCGTATTNAAGCCNNNNNNNNGCAAGATATCCATCACACTGG NCGGCCGCTCGAGCATGCATCTAGAGGGNCCCAATTTCNCCCCTAT RNA-9: no priming RNA-10: spliced EST, 99.0% >RNA-10-M13R_F10.ab1 NNNNNNNNNNNNGNNNNNNNNNNNNCNNNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTATNGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTCCTAGCTCATCCTGGCTCAAAAAGCACCCCCACTGA GCACCTTGCGACCCCCACTCCTGCCCGCCAGAGAACAAACCCCCTTTGACTGTAATTTTCCTTTACCTACCCAAATCCTA TAAAACAGCTCCACCCCTATCTCCCTTCACTGACTCTCTTTTCGGACTCGGCCCCCCTGCACCCAGGTGAAATAAACAGC CATGTTGCTCACACAAAGCCTGTTTGGTGATCTCTTCACATGGATGCACATGAAATTTGGTGCTGTGACTCAAATCGGGG GACCTCCCTTGGGAGATCAATCCCCTGTACTCCTGTTCTTTGCTCCATGACAAAGATCCACCTATGACCTCAGGTCCTCA GACCAACCANCCCAAGGAACATCTCACCGATTGTAAATCAGGTGAAGTATGAAGTTATGAGGAGAAAGGAACAGAGGACA ACCACACAGAGAAAGTGTAGCTTTATCTACTAGAGCTGAAGACATTCAAATTTATGATCCAACAATTCTACTCTGAAGTG CAGTGGTGNATATACTGCAACAATGTGTACTCATGTGCCCTGAGAACTANNNNTGAAAAAAACCTTACAGCAATATGCNT AATANNNNNNAAAAAAAAAAAAAAAAAAAANGGGATNCCCCCTATNGNNGNNTCNNNNTTAAAGCCAAATTCTGGCNGAN ANTNCNTCANN RNA-11: PQ, CMTM3, 97.5% >RNA-11-M13R_F11.ab1 NNNNNNNNNNNNNNNNNNNANCNNNGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTATACGACTCACTAT AGGGGTANCCTTTTTTTTTTTTTTTTTTTTTTTTTGTCAGTCTGGCTTAATTCATTATGGCTTTTTTTCAGGGTAAGAAA TTCACACCCAAGAGAATAAGGCTACAAATGCTCCAAACATGGGTCCAGAACTCCGGCTCTTTTGTGAGGCCTAGAGTCAC AAAAAATGCCATCTGTTAAAAAGCTGCCTTTGCATAAAAAAATATAAAAAGTTACAAAATTCCATCAAAGACAGATACAC AGTATAAATCGTTAGGTGAACACAGAAAGCTGGTCTCATCTCCTGCAGTGGCCCATAAACCTGCCCTGANGTCTGGAAAT TCACTGTCCTGAGGGTCCAGGAACTCCTGCCTGCTGGCTGGGCTCTAGGAAGAAGGAGGGTTTGGCTGGGCCTGCCCAGC TCCCCATGACACTGTCACTGTGATGCCACTGTTGTACCCTCTGTCTTCTGGACCAGTCAGTCTATGAGCTCTTCTTTGAA ACAGTCGTAGACCCAGAATGGTCATCTCACCCCCAGAANACATGGTTTCCAAGGGTGGGAGGTGTTAGTTATGGGTGCCG AGCCCCACTTCCTGGGCGACAAGGCAAAAGGTCAATTTCCCCCTGCTTCAAGAATGAAGAGGGAACTGAAAAAAAAAAAA AAAAAAAAAAAAAAGGATACCCCTAANANTGAGTCNTATTAAANCCGAATTCTGCAANAATCCATNACNANTGGNGGCNG CTCGAANNNGCATTTAGAGGGNCCCNATNTCGCCCCTATANTGAGGTCNNAATANNAANTCACTGGCCCNTCNTTTTAAC AACNNTCNNTGNANNGGGAAAAACCCTGGCNGTTANCCCAATNTTNANTCGCCCTTGNAAGCANNNNCCCCCCTTTTNCC AGNCTGGGNGTAATAACCGAAAAGNNCCCNCANCNNNNCGCCCNTTCCCNANAGTTGCGCNACCNGANANNGNNAATGGG NCNGCNCCCNGNNNNCGGCGNNNTTATANNNNNGNNNNNNNNGN RNA-12: PQ, HN1, 98.9% >RNA-12-M13R_F12.ab1 NNNNNNNNNNNNNNGNNNNNNNNNNTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTNNNAGGNGATNNTTTTTTTTTTTTTTTTTTTTTTTTTTTACNNGTCCTAAACTACTAAACCTGCATTAAAAATTTCG GTTGGGGCGACCTCGGANCAAAACCCAACCTCCGANCAGTACNTGCTAAGACTTCACCAGTCAAAGCGAACTACTATACT CAATTGATCCAATAACTTGNCCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATTCTATTCTAGAGTCCATATCAAC AATAGGGTTTACGACCTCNATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT AAAGTCCTACGTGATCTGAGTTCAGACCGGAGNAATCCNNGTCGGTTTNTATCTACTTCAAATTCCTCCCTGTACGAAAG GACAAGAGAAATAAGGNCTACTTCACATAGNGCCTTCCCCCGTAAATGANATCATCTCAACTTAGTATNNTACCCACACC C RNA-13: no priming RNA-14: PQ, spliced TVC (truncated?) 87.6% >RNA-14-M13R_G02.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAGTACTGTGCTTTTATTAAAAAACAAAACAAAACAA AAAAAATACAATCAGGTACTGTCCAGACANGTTTGGGAAGGGAAAATCTCTTTTAAAGTCCTCAGTTTTCATCATCACCA TCACCATTATTATATTAATAATATTAATGTCCTTGAAATTTAAACAATATCACTTTTCTGGCTTCAGTTGTAGGAAATGT ACAAAACATCGAGGGTGGGGGTGGGGCAAGGGGTAACTTCCAATGACACAAGTTTCATCTTTGTGACCTCAACCAATAAC AATTGNACAGCTTTGTGTAAAACACAGCTGGCTCGGAGAATGACCATGTCCCCTGTATGGCCAGCTTGCCCCTCTCCCAT TCCCAGAGCCACCCAAGAGAAGTAAAATTCATGGTGTTGCTGTCACTAGGAGACTTTGCTCAATCGAACAAAAAAGTAGA AACTTGTAGACTGTTGTCTTTGGTGCAAAGGAAGTCGTTGCAGAGGGGCCTACCTCAGTGCCTCAGTGATCCGTGTGGTC TGCTTGGACGCCCATCAAATGCCCAAGGAGAGCTTCNNTGTNNNNNNTGGNTGTGTCGATGCTTTGGNAAAAATCCTTTC TTTTCTCTGGGGCCCCATTCCCTCTCTCACCNAATTATCCCTGGAATTTATGCCCNGCTTACGCCTGCCNTTCNANATTT CGGGAAGTTGCTGGGTNCTCAANTTTCANCTCACAAACTGGANTTGAGTACTCATGGCCCNTTNCAANNGGNAANCNAGT NTAANGCCCCTGACTGTNNATTATNGNNCCATATCTGCCCCATCAGGNNNCNCGATGACANTTCNNNAAAAAAAAAAAAA AAANNANAANAAAAANNGTNACCCCCTANNNNNAGTCTANTTTAAAGCCCNAATTCTCNGCNAAATANNNNTNNNNNNNG CNN RNA-15: spliced and truncated PCNA, 88.4% >RNA-15-M13R_G03.ab1 NNNNNNNNNNNNNNNNNNNNNNGNNTCGGANCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTANNNGGGNTCCTTTTTTTTTTTTTTTTTTTTTTTTACTTTNAAANTTGTTAACTTTATTTAAATTGTACAATATTTC TTAAACTGCATTTAGCGTCAAGATCNNNNNNGAGAATCACCAGTATTTGTAAAGCAGAAACATTCATGTTAACAAAACTA GTTTTTACTTTGGAGCTTATTCTTTAAGCAAATTATGGAGAATGGAGAGAAAAGAAAGTAGGTTATTTACAGAAGATATC TACATATTTTATTTTAATATTTAAGAATACTCAGGTACAAACTTGGTGACAGAAAAGACCTCAGGACACGCTGGCATCTC AGGAGCAATCTTCAAAGGTTTAGTTTTCTCAATTTCTAGCAATGCCTAAGATGCTTCCTCATCTTCAATCTTGGGAGCCA AATAATACTTTAAGTGTCCCATGTCAGCAATTTTATACTCTACAACAAGGGGCACATCTGCAGACATACTGAGTGTTACT GTTGNAGACAGTGGAGTGGCTTTTGTGAAAAAGTTCAGGTACCTCANAGCAAACGTTAGGTGAACAGGCTCATTCATCTC TATGGTTACCGCCTCCTCTTCCTTATCCACATTGCTTGTTTGTGACAACTNCCATGTTCCCGTTGCCAAGCTCTCCACTT GAAGAAAACTTAAAAAAAAAAAAAAAAAAAAAAGGATANCCCTANANNGAGTCNNATTTAAAGCCNAATTCTGCANATNN
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