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==Objective== *I compare DAPI-staining performance on fixed(EtOH) and non-fixed E.coli cells(using the old E.coli sample stored from 03-10-'10 in 4C). The fixed cell still give me bright DAPI signal, but the non-fixed E.coli cell showed very dim single as previous tests I did. *I start doubting if the E.coli samples in the non-fixed samples are too old or the DNA were degraded in these cells. **When bacteria are dying the DNA will be degraded slowly, however I thought the degradation is too slow to be regarded as a concern. *I grew the fresh E.coli cell on 04-25-'11, and will compare fresh cells with old cells on DNA dye staining again.
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