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==Background== *New samples were picked from sent to Sara for shearing. I received these samples today (11-04-'10). **Sample preparation link: http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-02_exp2 *Special notice: 1. No 2nd MDA for short-amplified amplicons to avoid the over-dose N6 primer. 2. '''In order to reduce the possible effect from tRNA, I reduced the amount of added tRNA carrier. For short-amplified samples (40~50ng DNA) only 350ng tRNA carrier were added.''' 3. Samples are diluted in 50uL(total volume) for shearing. Since 100uL sample is very full for Covaris microtubes (50uL in microtubes works fine in previous experiments).
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