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====Mme I digestion==== Qiaquick purified DNA 26ul x 34ng/ul 10X NEBuffer 4: 6ul 1mM SAM 2ul Mme I (2U/ul) 5ul H2O 21ul 37C 90min -> 80C 20min -> 4C hold. I ran the digestion in 6% PAGE gel without purification, it turned out that the DNA migration pattern was totally off, probably due to DNA:protein interaction.
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