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====Notes==== *22 images 170um away from each other **184umx184um FOV so ~14um (<10%) overlap between images *By the time I finish defining z-start and z-end positions the stage has drifted **Seems like stage drifts down ~3um per hour **Need to define >3um below what I want and leave extra room above and below *Left sample overnight and it dried out (gel was all cracked) **Rehydrated in 2X SSC for 1hr ***Tissue morphology looks the same ***Fewer/less rolony signal but this could be due to 2 other factors *20160810_S2_BA8_DARTFISH_TB12kApr2016V4_Cycle# **Cycle0 = Universal rolony probe (Cy3) **Cycle1-6 for decoding **Cycle7 for DRAQ5 *Pos1 of 22 with approx position of tissue section outlined in red [[File:20160810_22pos_DARTFISH_BA8_Pos1.JPG.JPG|350px]] *Pos22 of 22 with approx position of imaged tile outlined in red [[File:20160810_22pos_DARTFISH_BA8_Pos22.JPG|350px]] =====Cycle2===== *Retook Position19019 and Position20020 because z range was offset, started from middle of signal =====Cycle6===== *Retook Position20020 and Position21021 because z range was offset, started from middle of signal
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