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===== Compare the gel image ===== '''no pre-amplification''' [[File:ZhangLab_2 2014-06-03 16hr 20min_V1N3_1000to1.jpg| 420px]] '''Pre-amplification''' [[File:ZhangLab_2 2014-06-06 14hr 13min_V1N3_CANC2-5-50ng-pream.jpg| 420px]] * From the gel images, it looked like the intensity of BSPP capture with pre-amplification is higher than no pre-amplification, especially 10ng input. For 5ng, the reactions with pre-amp seemed to be a little brighter than no-amp. * However, there is more background in pre-amp capture. I guess this might be decrease with 1-2 less cycle numbers. * I should have done these experiments side by side to confirm that a smear band did not come from the original BIS-DNA template as without amplification, the capture worked. '''Gel image of BSPP capture of pre-amplified DNA without column purification before ecapture'''<br> '''experiment on 2014-04-10''' [[File:ZhangLab_2 2014-04-10 18hr 50min_GP1-V4-gel2_ee.png| 450px]]<br>
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