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===Decoding Sample 2: Image Analysis=== *331 GB total size of raw images *Due to time constraints during imaging, could not export raw tiff files from Leica software during imaging process **Each cycle would need >30min to export and I already use all the downtime to save the experiment in Leica format #So first thing to do was reopen each experiment in Leica and then export as tiff #*Each cycle saved as its own experiment because Leica software has limited memory #*Each cycle takes about 2 hours to open and then export #Then uploaded raw data from local computer to ConfocalStore server #Then downloaded from ConfocalStore server to my laptop's external hard drive #Replaced Cycle2 Pos19 & Pos20 and Cycle6 Pos20 & Pos21 with retaken images #*i.e. replace 20160810_S2_BA8_DARTFISH_TB12kApr2016V4_Cycle2\Position19019\ with 20160810_S2_BA8_DARTFISH_TB12kApr2016V4_Cycle2_Pos19-20\Position19019\ #First try 2D decoding because much faster #Make Max Intensity Z Projection for every stack #*[[Media:BatchMIP.txt|BatchMIP.py]] <!-- ==Results== -->
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