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==Discussion== The following emailed to Paola: #All the sections have a thin layer of tissue inside the ring. So the rings are ~20um thick but the tissue inside the ring is <5um thick. This middle has a lot of rolonies too. #Noticeable difference between A1 and A2. All the A1 sections stayed on the glass and showed much more rolonies. I'm wondering if this is a difference between your sectioning technique or how I fixed them. #*One section was in direct contact with metal of 37C incubator while the other was not during fixation. #How do you think the tissue looks? Maybe you remember which order you cut them in and can relate that to their result.
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