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===Clinical sample and DNA collection=== *NSCLC samples and corresponding normal lung tissues for validation study in Chinese population were obtained from 150 patients who underwent pulmonary resection for primary NSCLC at Changhai Hospital, Shanghai, China. The study was approved by Fudan University and Changhai Hospital and Informed consents were obtained from the patients. Exclusion criteria included subjects with a family history of lung cancer, previous radiotherapy, and chemotherapy or adjuvant therapy before surgery. All tissues were immediately frozen at -80β after surgical resection. Histological examination and tumor-node-metastasis classification were conducted according to World Health Organization classification criteria (5) and AJCC Cancer Staging Manual, 7th Edition (6), respectively. Age, gender, smoking status, histology type, TNM stage and differentiation status were collected as the covariates when conducting association between DNA methylation and disease status. Smoking status was assigned to binary status: never and ever smoking. TNM stage was assigned to early stage (I and II) or late stage (III and IV) when it is necessary so that the sample size can be big enough to get the efficient statistic power. * Plasma ** 10 PC-P, Noi: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-6-9 *CTT-frozen and CTT-FFPE RRBS library and sequencing [http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-6-2] ** The aim of expriments on TT-frozen and CTT-FFPE * [[N37 samples]] with WGBS Bam files were stored in TSCC server:
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